support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: FosB
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 2354
Swiss Prot: P53539
Source: Recombinant human FosB protein, around 1-150aa.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Size: 100µL
Concentration: 1ug/ul
Applications:
WB(1:1000-2000)
IHC-P(1:50-200)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
4% Paraformaldehyde-fixed Hela (H) cell (TPA stimulated); Triton X-100 at r.t. for 20 min; Antibody incubation with (FosB) monoclonal Antibody, unconjugated (bsm-52071R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
NIH/3T3 (M) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h,C6 (R) cells were treated with or without serum-starved overnight and serum-stimulated for 4 h, 25 μg total protein per lane of cell lysates (see on figure) probed with FosB monoclonal antibody, unconjugated (bsm-52071R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.