VALIDATION IMAGES
293 cells were stained with HDAC6 (6B4) Monoclonal Antibody (bsm-52086R) at 1:200 dilution, incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with HDAC6 (6B4) Monoclonal Antibody (bsm-52086R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: COS-7, 3: K562 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 131 kDa Observed MW: 160 kDa
Tissue: Human kidney Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52086R
Tissue: Human testis Section type: Formalin-fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:50 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-52086R