bsm-52112R [Primary Antibody]
Histone H4 (tri methyl K20) (2C12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H4 (tri methyl K20)

Modification Site: tri methyl K20

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 121504

Swiss Prot: P62805

Source: Synthetic peptide derived from human Histone H4 (tri methyl K20), around 1-30aa (tri methyl K20).

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage Condition: Store at -20°C for 12 months.

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Histone H4 (tri methyl K20) (2C12) Monoclonal Antibody (bsm-52112R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.