DATASHEET
Host:
Rabbit
Target Protein:
Histone H4 (acetyl K5)
Modification Site:
acetyl K5
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
121504
Swiss Prot:
P62805
Source:
Synthetic peptide derived from human Histone H4(acetyl K5), around 1-30aa (acetyl K5).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].
VALIDATION IMAGES
Lane 1: CRC Cell lysates; Lane 2: HeLa Cell lysates; probed with Histone H4(acetyl K5) (1G3) Monoclonal Antibody (bsm-52113R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HeLa cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Histone H4(acetyl K5) (1G3) Monoclonal Antibody (bsm-52113R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Histone H4(acetyl K5) (1G3) Monoclonal Antibody (bsm-52113R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.