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Host: Rabbit
Target Protein: IRF1
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3659
Swiss Prot: P10914
Source: Recombinant human IRF1 protein, around C-terminal 100aa
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Predicted Molecular Weight: 37
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
- Pengcheng Zhou. et al. Tiao-bu-fei-shen formula promotes downregulation of the caveolin 1-p38 mapk signaling pathway in COPD - Associated tracheobronchomalacia cell model. J ETHNOPHARMACOL. J Ethnopharmacol. 2022 Apr;:115256Read more>>
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with IRF1 (3G7) Monoclonal Antibody (bsm-52114R) at 1:100 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: Human THP-1 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MOLT4 cell lysates probed with IRF1 Monoclonal Antibody, Unconjugated (bsm-52114R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.