VALIDATION IMAGES
Lane 1: Hela; Lane 2: Jurkat; Lane 3: THP-1 lysates probed with IRF3 (4C3) Monoclonal Antibody (bsm-52116R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .
MCF-7 cells were stained with IRF3 (4C3) Monoclonal Antibody (bsm-52116R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with IRF3 (4C3) Monoclonal Antibody (bsm-52116R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: Hela cell lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse Lymph node lysates; Lane 4: A549 cell lysates probed with IRF3 Monoclonal Antibody, Unconjugated (bsm-52116R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human 293T cell lysates probed with IRF3 Monoclonal Antibody, Unconjugated (bsm-52116M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.