DATASHEET
Host:
Rabbit
Target Protein:
Peroxiredoxin 1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
5052
Swiss Prot:
Q06830
Source:
Recombinant human Peroxiredoxin 1 protein, full length.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
VALIDATION IMAGES
Lane 1: Hela; Lane 2: A431 lysate probed with Peroxiredoxin 1 (7A1) Monoclonal Antibody, Unconjugated (bsm-52127R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Peroxiredoxin 1 (7A1) Monoclonal Antibody (bsm-52127R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Peroxiredoxin 1 (7A1) Monoclonal Antibody (bsm-52127R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.