bsm-52127R [Primary Antibody]
Peroxiredoxin 1 (7A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Peroxiredoxin 1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5052

Swiss Prot: Q06830

Source: Recombinant human Peroxiredoxin 1 protein, full length.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela; Lane 2: A431 lysate probed with Peroxiredoxin 1 (7A1) Monoclonal Antibody, Unconjugated (bsm-52127R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


Paraformaldehyde-fixed, paraffin embedded human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Peroxiredoxin 1 (7A1) Monoclonal Antibody (bsm-52127R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Peroxiredoxin 1 (7A1) Monoclonal Antibody (bsm-52127R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.