Host: Rabbit
Target Protein: Phospho-Akt1 (Ser473)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 207
Swiss Prot: P31749
Source: KLH conjugated Synthesised phosphopeptide derived from human Akt1 around the phosphorylation site of Ser473
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Predicted Molecular Weight: 56
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: Human Jurkat cell lysates; Lane 2: Human MOLT4 cell lysates probed with Phospho-Akt1(Ser473) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-Akt1 (Ser473) Monoclonal Antibody, Unconjugated(bsm-52130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Lane 1: Human HeLa cell lysates; Lane 2: Human Hela treated with 100nM Calyculin A for 30min; Lane 3: Human MCF-7 cell lysates; Lane 4: Human MCF-7 treated with 100nM Calyculin A for 30min probed with Phospho-Akt1 (Ser473) Monoclonal Antibody, Unconjugated (bsm-52130R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-Akt1 (Ser473) Monoclonal Antibody, Unconjugated(bsm-52130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-Akt1 (Ser473) Monoclonal Antibody, Unconjugated(bsm-52130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-Akt1 (Ser473) Monoclonal Antibody, Unconjugated(bsm-52130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-Akt1 (Ser473) Monoclonal Antibody, Unconjugated(bsm-52130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.