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Host: Rabbit
Target Protein: Phospho-Akt1 (Ser473)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 207
Swiss Prot: P31749
Source: KLH conjugated Synthesised phosphopeptide derived from human Akt1 around the phosphorylation site of Ser473
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications: WB(1:1000-5000)
Predicted Molecular Weight: 56
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 DecRead more>>
- Yaji Li. et al. CircMYBL1 suppressed acquired resistance to osimertinib in non-small-cell lung cancer. CANCER GENET-NY. 2024 Jun;284-285:34Read more>>
Hela (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-Akt1 (Ser473) monoclonal antibody, unconjugated (bsm-52130R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
MCF-7 (H) cells were treated with or without Calyculin A (100nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-Akt1 (Ser473) monoclonal antibody, unconjugated (bsm-52130R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.