Host: Rabbit
Target Protein: c-Jun(S63)
Modification Site: S63
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 3725
Swiss Prot: P05412
Source: Synthetic peptide derived from human c-Jun(S63), around 50-100aa(phospho S63).
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1: NIH/3T3; Lane 2: 293T lysates probed with c-Jun(Ser63) (6C2 ) Monoclonal Antibody (bsm-52141R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
ICC staining of c-Jun(S63) in MCF-7 cells (green) using c-Jun(S63) (6C2) Monoclonal Antibody (bsm-52141R). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
ICC staining using c-Jun(S63) (6C2) Monoclonal Antibody followed by A549 conjugated secondary antibody staining (green), and nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed, paraffin embedded Human laryngeal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human skin cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human esophageal carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with c-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human SH-SY5Y cell lysates; Lane 4: Human U251 cell lysates probed with c-Jun(S63) (6C2) Monoclonal Antibody, Unconjugated (bsm-52141R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.