bsm-52159R [Primary Antibody]
Glycogen synthase 1(S641) (10C1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Glycogen synthase 1(S641)

Modification Site: S641

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 2997

Swiss Prot: P13807

Source: Synthetic peptide derived from human Glycogen synthase 1(S641), around 630-680aa (phospho S641).

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Glycogen Synthase (GS) is a key enzyme in the regulation of glycogen metabolism. GS catalyzes the incorporation of UDP-glucose incorporation into glycogen. The activity of glycogen synthase is regulated by hormonal stimuli (insulin, catecholamines and glucagons) and non-hormonal stimuli (blood glucose level and exercise). Two main isoforms of mammalian GS are designated as muscle (glycogen synthase 1) and liver (glycogen synthase 2). Most tissues express glycogen synthase 1, whereas glycogen synthase 2 appears to be tissue-specific. The two isoforms have 70% identical amino acid sequence. Glycogen synthase can be phosphorylated by multiple kinases including glycogen synthase kinase-3 (GSK-3), mitogen-activated protein kinase-related protein kinase (DYRK), and SAPK2b/p38b which leads to its inactivation.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Aphidicolin treated Mouse liver lysates; Lane 2: Untreated Mouse Liver lysates probed with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody (bsm-52159R) at 1:1000.


A549 cells were stained with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody (bsm-52159R) at 1:200 followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded mouse skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody (bsm-52159R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Glycogen synthase 1(S641) (10C1) Monoclonal Antibody, Unconjugated (bsm-52159R) at 1:200 overnight at 4°C, DAB staining.