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Host: Rabbit
Target Protein: HSF1 (Ser326)
Modification Site: S326
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 3297
Swiss Prot: Q00613
Source: KLH conjugated Synthesised phosphopeptide derived from human HSF1 around the phosphorylation site of Ser326
Purification: purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(1:500-2000)
IHC-F(1:100-500)
IF(1:100-500)
Flow-Cyt(1:50-100)
IHC-P(1:100-500)
Predicted Molecular Weight: 57 kDa
Cross Reactive Species: Human
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSF1 (S326) (37E4) Monoclonal Antibody, Unconjugated (bsm-52166R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSF1 (S326)) Monoclonal Antibody, Unconjugated (bsm-52166R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HeLa (H) treated with heat at 43℃ for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-HSF1 (Ser326) monoclonal antibody, unconjugated (bsm-52166R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSF1 (Ser326) Monoclonal Antibody, Unconjugated (bsm-52166R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSF1 (Ser326) Monoclonal Antibody, Unconjugated (bsm-52166R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSF1 (Ser326) Monoclonal Antibody, Unconjugated (bsm-52166R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-HSF1 (Ser326) Monoclonal Antibody, Unconjugated (bsm-52166R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.