bsm-52168R [Primary Antibody]
Hsp27 (Ser82) Monoclonal Antibody
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Host: Rabbit

Target Protein: Hsp27 (Ser82)

Modification Site: Ser82

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3315

Swiss Prot: P04792

Source: Synthetic peptide derived from human Hsp27(S82), around 50-100 amino acids.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal Alpha-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela (H) cells were treated with or without UV (40 mJ/cm2) for 30 min and anisomycin (25 μg/mL) for 30 min , 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-HSP27 (Ser82) monoclonal antibody, unconjugated (bsm-52168R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.