DATASHEET
Host:
Rabbit
Target Protein:
IKB alpha (Ser32)
Modification Site:
Ser32
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
4792
Swiss Prot:
P25963
Source:
Synthetic peptide derived from human IKB alpha (Ser32) around 1-50 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
PRODUCT SPECIFIC PUBLICATIONS
- Jingwei Song. et al. Swine MicroRNAs ssc-miR-221-3p and ssc-miR-222 Restrict the Cross-Species Infection of Avian Influenza Virus. J Virol. 2020 Nov;94(23):e01700-2Read more>>
- Mei-Hua Jin. et al. Hispidin inhibits LPS?induced nitric oxide production in BV?2 microglial cells via ROS?dependent MAPK signaling. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
- Tian, Mengyue. et al. The protective effect of licochalcone A against inflammation injury of primary dairy cow claw dermal cells induced by lipopolysaccharide. Sci Rep-Uk. 2022 Jan;12(1):1-9Read more>>
- Yi Zhirong. et al. Elian granules alleviate precancerous lesions of gastric cancer in rats by suppressing M2-type polarization of tumor-associated macrophages through NF-B signaling pathway. BMC Complementary Medicine and Therapies. 2023 Dec;23(1):1-11Read more>>
- Yufei Jiang. et al. Gq-PKD/PKC signal regulating the nuclear export of HDAC5 to induce the IB expression and limit the NF-B-mediated inflammatory response essential for early pregnancy. ELIFE. 2023 JulRead more>>
VALIDATION IMAGES
Ags cell lysates probed with IKB alpha (Ser32) (13E7) Monoclonal Antibody (bsm-52169R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IKB alpha (Ser32) (13E7) Monoclonal Antibody(bsm-52169R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).