bsm-52170R [Primary Antibody]
IRF3(S386) (28C3) Monoclonal Antibody
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Host: Rabbit

Target Protein: IRF3(S386)

Modification Site: S386

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3661

Swiss Prot: Q14653

Source: Synthetic peptide derived from human IRF3(S386), around 350-400aa (phospho S386).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity. [provided by RefSeq].

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:500-2000)
ICC/IF(1:100-200)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shenjie Zhong. et al. Bmp8a is an essential positive regulator of antiviral immunity in zebrafish. Commun Biol. 2021 Mar;4(1):1-15Read more>>
  • Lihui Chen. et al. Bmp4 in Zebrafish Enhances Antiviral Innate Immunity through p38 MAPK (Mitogen-Activated Protein Kinases) Pathway. INT J MOL SCI. 2023 Jan;24(19):14444Read more>>
  • Jiawei Sun. et al. High Manganese Content of Lipid NanoMn (LNM) by Microfluidic Technology for Enhancing Anti-Tumor Immunity. PHARMACEUTICS. 2024 Apr;16(4):556Read more>>
VALIDATION IMAGES

MCF-7 (H) cells were treated with or without Calyculin A (100nM) for 45 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-IRF3 (Ser386) monoclonal antibody, unconjugated (bsm-52170R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.