bsm-52176R
[Primary Antibody]
Phospho-MEK1/2 (Ser218 + Ser222) Recombinant Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET
Host: Rabbit
Target Protein: Phospho-MEK1/2 (Ser218 + Ser222)
Modification Site: S218/S222
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 5604
Swiss Prot: Q02750
Source: Synthetic peptide derived from human MEK1(S218/S222), around 200-250aa (phospho S218+S222).
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage: Store at -20°C for 12 months.
Background:
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.
Size: 100µL
Concentration: Lot Dependent
Applications:
WB(WB(1:1000-2000))
IHC-P(IHC-P(1:50-200))
IHC-F(IF(ICC)(1:50-200))
IF
ICC/IF(1:50-100)
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yubing Yu. et al. Angiogenic factor AGGF1 Blocks Neointimal Formation after Vascular Injury via Interaction with Integrin _7 on Vascular Smooth Muscle Cells. J Biol Chem. 2022 Feb;:101759Read more>>
- Xing-Peng Di. et al. YAP/Smad3 promotes pathological extracellular matrix microenviroment-induced bladder smooth muscle proliferation in bladder fibrosis progression. MedComm. 2022 Sep;3(4):e169Read more>>
- Chen Cheng. et al. Nerve growth factor promote osteogenic differentiation of dental pulp stem cells through MEK/ERK signalling pathways. J CELL MOL MED. 2024 Feb;28(4):e18143Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-MEK1/2 (Ser218 + Ser222) Monoclonal Antibody, Unconjugated (bsm-52176R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Hela (H) cells were treated with or without hEGF (100ng/ml) for 5 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-MEK1/2 (Ser218 + Ser222) monoclonal antibody, unconjugated (bsm-52176R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.