bsm-52179R [Primary Antibody]
Nrf2(S40) (7G4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Nrf2(S40)

Modification Site: S40

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4780

Swiss Prot: Q16236

Source: Synthetic peptide derived from human Nrf2(S40), around 1-70aa (phospho S40).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zubeyir Elmazoglu. et al. Cannabinoid-profiled agents improve cell survival via reduction of oxidative stress and inflammation, and Nrf2 activation in a toxic model combining hyperglycemia+A1-42 peptide in rat hippocampal neurons. Neurochem Int. 2020 Nov;140:104817Read more>>
VALIDATION IMAGES

Lane 1: HepG2; Lane 2: Raji lysates probed with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


HepG2 cells were stained with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


A549 cells were stained with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded human tonsil section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Nrf2(S40) (7G4) Monoclonal Antibody (bsm-52179R) at 1:200, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.