bsm-52197R [Primary Antibody]
Phospho-Rb (Ser807) Recombinant Antibody
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Host: Rabbit

Target Protein: Phospho-Rb (Ser807)

Modification Site: S807

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 5925

Swiss Prot: P06400

Source: Synthetic peptide derived from human Rb(S807), around 800-850aa (phospho S807).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
ICC/IF(1:50-100)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Molin Li. et al. PPP3R1 Promotes MSCs Senescence by Inducing Plasma Membrane Depolarization and Increasing Ca2+ Influx. INT J MOL SCI. 2023 Jan;24(5):4421Read more>>
  • ang-Hua Zhong. et al. Transcription factor FOXF2 promotes the development and progression of pancreatic cancer by targeting MSI2. ONCOL REP. 2024 Jul;52(1):1-13Read more>>
VALIDATION IMAGES

Lane 1: A549 lysates probed with Rb(S807) (2D10) Monoclonal Antibody (bsm-52197R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody for 60 minutes.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-Rb (Ser807) Monoclonal Antibody, Unconjugated (bsm-52197R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.