DATASHEET
Host:
Rabbit
Target Protein:
Rb(S807)
Modification Site:
S807
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
5925
Swiss Prot:
P06400
Source:
Synthetic peptide derived from human Rb(S807), around 800-850aa (phospho S807).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Nuclear Marker.The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
PRODUCT SPECIFIC PUBLICATIONS
- Molin Li. et al. PPP3R1 Promotes MSCs Senescence by Inducing Plasma Membrane Depolarization and Increasing Ca2+ Influx. INT J MOL SCI. 2023 Jan;24(5):4421Read more>>
VALIDATION IMAGES
Lane 1: A549 lysates probed with Rb(S807) (2D10) Monoclonal Antibody (bsm-52197R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody for 60 minutes.
A549 cells were stained with Rb(S807) (2D10) Monoclonal Antibody (bsm-52197R) at [1:200] incubated for overnight at 4C, followed by secondary antibody incubation and DAPI staining.