bsm-52198R [Primary Antibody]
RSK1 p90(T359+S363) (8H2) Monoclonal Antibody
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Host: Rabbit

Target Protein: RSK1 p90(T359+S363)

Modification Site: T359+S363

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6195

Swiss Prot: Q15418

Source: Synthetic peptide derived from human RSK1 p90(T359+S363), around 300-400aa (phospho T359+S363).

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4).

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:1000-2000)
IHC-F(1:50-200)
IF(1:50-200)
Flow-Cyt(1:50-100)
IHC-P(1:50-200)
ICC/IF(1:50-200)

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: A431; Lane 2: 293; Lane 3: MCF-7 cell lysates probed with RSK1 p90(T359+S363) (8H2) Monoclonal Antibody (bsm-52198R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-RPS6KA1 (Thr359 + Ser363) Monoclonal Antibody, Unconjugated (bsm-52198R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.