bsm-52202R [Primary Antibody]
SIRT1 (Thr530) Recombinant Antibody
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Host: Rabbit

Target Protein: SIRT1 (Thr530)

Modification Site: T530

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 23411

Swiss Prot: Q96EB6

Source: KLH conjugated synthesised phosphopeptide derived from human SIRT1 around the phosphorylation site of Thr530

Purification: purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Size: 100ul

Concentration: Lot Dependent

Applications: WB(1:500-1000)

Predicted Molecular Weight: 81 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with phospho-SIRT1 (Thr530) monoclonal antibody, unconjugated (bsm-52202R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.