bsm-52203R [Primary Antibody]
Smad2 (S250) (1G2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Smad2 (S250)

Modification Site: S250

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4087

Swiss Prot: Q15796

Source: Synthetic peptide derived from human Smad2 (S250), around 220-280aa (phospho S250).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HeLa Cell lysates; probed with Smad2 (S250) (1G2) Monoclonal Antibody (bsm-52203R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.