bsm-52204R

DATASHEET

Host: Rabbit

Target Protein: phospho-Smad2 (Ser255)

Modification Site: S255

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4087

Swiss Prot: Q15796

Source: Synthetic peptide derived from human Smad2(S255), around 220-280aa (phospho S255).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(WB(1:1000)IHC(1:50-200))
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
ICC/IF(1:50-100)

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.