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Host: Rabbit
Target Protein: Phospho-Smad3 (Ser423 + Ser425)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 4088
Swiss Prot: P84022
Source: KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: Lot Dependent
Predicted Molecular Weight: 47
Cross Reactive Species: Human
Predicted Cross Reactive Species: Mouse
For research use only. Not intended for diagnostic or therapeutic use.
- Xiaoliang Zhou. et al. Ursolic acid inhibits human dermal fibroblasts hyperproliferation, migration, and collagen deposition induced by TGF- via regulating the Smad2/3 pathway. GENE. 2023 May;867:147367Read more>>
A549 (H) cells were treated with or without hTGF-β (10 ng/ml) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-Smad3 (Ser423 + Ser425) monoclonal antibody, unconjugated (bsm-52205R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.