bsm-52211R [Primary Antibody]
STAT3(Tyr705) Monoclonal Antibody
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Host: Rabbit

Target Protein: STAT3(Tyr705)

Modification Site: Tyr705

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6774

Swiss Prot: P40763

Source: Synthetic peptide derived from human STAT3(Tyr705), around 700-750aa (phospho T705).

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:1000-2000)ICC(1:50-200)
IHC(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hui Yan. et al. Elaboration a ROS-responsive darutigenol prodrug nanoassemblies for inflammatory arthritis treatment. NANO TODAY. 2024 Apr;55:10222Read more>>
VALIDATION IMAGES

Jurkat (H) cells were treated with or without IFN (50 ng/ml) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with phospho-STAT3 monoclonal antibody, unconjugated (bsm-52211R) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Tyr705) Monoclonal Antibody, Unconjugated(bsm-52211R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-STAT3 (Tyr705) Monoclonal Antibody, Unconjugated(bsm-52211R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) Monoclonal Antibody, Unconjugated(bsm-52211R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) Monoclonal Antibody, Unconjugated(bsm-52211R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.