support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: Phospho-YAP1 (Ser127)
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 10413
Swiss Prot: P46937
Source: A synthesized peptide derived from human YAP1 around the phosphorylation site of S127
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB(1:500-5000))
IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254Read more>>
- Zhao, Boyuan. et al. Suspension state and shear stress enhance breast tumor cells EMT through YAP by microRNA-29b. 2021 Oct 07Read more>>
- Manlin He. et al. Polydatin attenuates tubulointerstitial fibrosis in diabetic kidney disease by inhibiting YAP expression and nuclear translocation.. FRONT PHYSIOL. 2022 Oct;13:927794-927794Read more>>
- Lan Feng. et al. Dapagliflozin delays renal fibrosis in diabetic kidney disease by inhibiting YAP/TAZ activation. LIFE SCI. 2023 Jun;322:121671Read more>>
- Yang Xi. et al. circ0005027 Acting as a ceRNA Affects the Malignant Biological Behavior of Hypopharyngeal Squamous Cell Carcinoma by Modulating miR-548c-3p/CDH1 Axis. BIOCHEM GENET. 2023 Nov;:1-16Read more>>
- Peiqi Liu. et al. Mild heat stress promotes the differentiation of odontoblast-like MDPC-23 cells via yes-associated protein. INT J HYPERTHER. 2024 26Read more>>
Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated(bsm-54797R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated(bsm-54797R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated(bsm-54797R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated(bsm-54797R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated(bsm-54797R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Duodenum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-YAP1 (Ser127) Monoclonal Antibody, Unconjugated(bsm-54797R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
NIH/3T3 (M) cells were treated with or without Calyculin A (100ng/ml) for 30 min, Hela (H) cells were treated with or without Okadaic A+ calyculin A (100 nM) for 60 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-YAP1 monoclonal antibody, unconjugated (bsm-52214R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.