DATASHEET
Host:
Rabbit
Target Protein:
YAP1 (S127)
Modification Site:
S127
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
10413
Swiss Prot:
P46937
Source:
Synthetic peptide derived from human YAP1 (S127), around 100-150aa (phospho S127).
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
YAP1 (also known as Yes associated protein 1) was originally identified as a transcription factor that binds to the SH3 domain of the YES kinase (a Src protein kinase). More recently it has been identified as a candidate oncogene that promotes tumorigenesis in many different types of cancer.
PRODUCT SPECIFIC PUBLICATIONS
- Qi Yang. et al. A novel biodegradable external stent regulates vein graft remodeling via the Hippo-YAP and mTOR signaling pathways. Biomaterials. 2020 Nov;258:120254Read more>>
- Zhao, Boyuan. et al. Suspension state and shear stress enhance breast tumor cells EMT through YAP by microRNA-29b. 2021 Oct 07Read more>>
- Manlin He. et al. Polydatin attenuates tubulointerstitial fibrosis in diabetic kidney disease by inhibiting YAP expression and nuclear translocation.. FRONT PHYSIOL. 2022 Oct;13:927794-927794Read more>>
- Lan Feng. et al. Dapagliflozin delays renal fibrosis in diabetic kidney disease by inhibiting YAP/TAZ activation. LIFE SCI. 2023 Jun;322:121671Read more>>
- Yang Xi. et al. circ0005027 Acting as a ceRNA Affects the Malignant Biological Behavior of Hypopharyngeal Squamous Cell Carcinoma by Modulating miR-548c-3p/CDH1 Axis. BIOCHEM GENET. 2023 Nov;:1-16Read more>>
VALIDATION IMAGES
MCF7 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with YAP1 (S127) (1H12) Monoclonal Antibody(bsm-52214R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).