bsm-52215R [Primary Antibody]
PI 3 Kinase p55 gamma (6A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PI 3 Kinase p55 gamma

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8503

Swiss Prot: Q92569

Source: Recombinant human PI 3 Kinase p55 gamma protein, around 350-460aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: MCF-7 lysates; Lane 2: Jurkat lysates; Lane 3: Mouse testis lysates probed with PI 3 Kinase p55 gamma (6A1 ) Monoclonal Antibody (bsm-52215R) at 1:1000.


Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with PI 3 Kinase p55 gamma (6A1) Monoclonal Antibody (bsm-52215R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


PC-3M cells were stained with PI 3 Kinase p55 gamma (6A1) Monoclonal Antibody (bsm-52215R) at 1:200.