DATASHEET
Host:
Rabbit
Target Protein:
PI 3 Kinase p85 alpha
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
5295
Swiss Prot:
P27986
Source:
Recombinant human PI 3 Kinase p85 alpha protein, around 600-700aa.
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.
VALIDATION IMAGES
Lane 1: MCF-7; Lane 2: Raji lysate probed with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody .
HeLa cells were stained with PI 3 Kinase p85 alpha (2G4) Monoclonal Antibody (bsm-52216R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human MOLT-4 cell lysates; Lane 8: Human HL-60 cell lysates probed with PI 3 Kinase p85 alpha Monoclonal Antibody, Unconjugated (bsm-52216R ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.