Recombinant human Smad1 protein, around N-terminal 1-200aa.
Purified by Protein A.
Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq].
Lane 1: Human skeletal muscle; Lane 2: Hela; probed with HDAC2 (3B7) Monoclonal Antibody (bsm-52082R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
MCF-7 cells were stained with Smad1 (2D9) Monoclonal Antibody (bsm-52222R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Lane 1: Human Huvec cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MCF-7 cell lysates probed with Smad1 Monoclonal Antibody, Unconjugated (bsm-52222R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.