bsm-52234R [Primary Antibody]
STAT2 (4B1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: STAT2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6773

Swiss Prot: P52630

Source: Recombinant human STAT2 protein, around N-terminal 1-100aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A431 cells were fixed in paraformaldehyde and permeabilized with 0.25% Triton X100/PBS. Antibody incubation with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100 for 1hr at 37°C, followed by a conjugated secondary antibody and DAPI for nuclei staining


Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue was incubated with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counter stained with hematoxylin


Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue was incubated with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin


Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue was incubated with STAT2 (4B1) Monoclonal Antibody, Unconjugated (bsm-52234R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counter stained with hematoxylin