bsm-52235R [Primary Antibody]
Stat3 (6G5) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Stat3

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6774

Swiss Prot: P40763

Source: Recombinant human Stat3 protein, around N-terminal 1-100aa.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hongbing Zhou. et al. Expression of STAT3 and vasculogenic mimicry in gallbladder carcinoma promotes invasion and metastasis. Exp Ther Med. 2021 Jul;22(1):1-1Read more>>
  • Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692Read more>>
  • Mei-Hua Jin. et al. Hispidin inhibits LPS?induced nitric oxide production in BV?2 microglial cells via ROS?dependent MAPK signaling. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
  • Yuxi Jia. et al. Effects of Dietary n-3/n-6 PUFA Ratios on Animal Behaviors and Inflammation in a Prenatal Valproic Acid Exposure-Induced Rat Model of Autism. Neuropsych Dis Treat. 2022 Feb;18:401-412Read more>>
  • Yong Wang. et al. An integrated network pharmacology approach reveals that Darutigenol reduces inflammation and cartilage degradation in a mouse collagen-induced arthritis model by inhibiting the JAK-STAT3 pathway. J ETHNOPHARMACOL. 2023 May;:116574Read more>>
  • Qingming Bao. et al. Tumor targeted siRNA delivery by adenosine receptor-specific curdlan nanoparticles. INT J BIOL MACROMOL. 2023 Dec;253:126845Read more>>
VALIDATION IMAGES

Lane 1: HeLa lysates; Lane 2: SHG-44 lysates; Lane 3: SW480 Lysates; probed with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


CRC cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Stat3 (6G5) Monoclonal Antibody (bsm-52235R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Stat3 Antibody(bsm-52235R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Hela cell lysates; Lane 2: A431 cell lysates probed with Stat3 Monoclonal Antibody, Unconjugated (bsm-52235R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.