bsm-52239R [Primary Antibody]
STAT6 (9A7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: STAT6

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6778

Swiss Prot: P42226

Source: Recombinant human STAT6 protein, around 700-800aa.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xue-yin Pan. et al. Alternative activation of macrophages by prostacyclin synthase ameliorates alcohol induced liver injury. Lab Invest. 2021 Jun;:1-15Read more>>
VALIDATION IMAGES

Lane 1: NIH/3T3 lysates; Lane 2: Hela lysates probed with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.


Hela cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS and stained with STAT6 (9A7) Monoclonal Antibody (bsm-52239R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.


Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with STAT6 (9A7) Monoclonal Antibody at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.


A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with STAT6 Antibody(bsm-52239R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human Hela cell lysates probed with STAT6 monoclonal Antibody, Unconjugated (bsm-52239R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.