bsm-52245R
[Primary Antibody]
CREB (2B5 ) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CREB
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P16220
Source: Human CREB from 50 to 200 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela; Lane 2: HepG2; Lane 3: Jurkat; Lane 4: NIH/3T3; Probed with CREB (2B5 ) Monoclonal Antibody (bsm-52245R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of HeLa cells with CREB (2B5 ) Monoclonal Antibody (bsm-52245R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
Zebrafish lysate probed with CREB (2B5 ) Monoclonal Antibody (bsm-52245R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with CREB (2B5) Monoclonal Antibody (bsm-52245R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CREB (2B5) Monoclonal Antibody (bsm-52245R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded human lung cancer tissue incubated with CREB (2B5) Monoclonal Antibody (bsm-52245R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded human breast carcinoma tissue incubated with CREB (2B5) Monoclonal Antibody (bsm-52245R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CREB (2B5) Monoclonal Antibody (bsm-52245R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.