bsm-52249R
[Primary Antibody]
PBR (1D4) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PBR
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 706
Swiss Prot: P30536
Source: Human PBP between 50-150 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme (By similarity). Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed:1847678).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: 293T; Lane 2: NIH/3T3; Lane 3: HepG2 lysates probed with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:1000 dilution and incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of HeLa cells with PBR (1D4) Monoclonal Antibody (bsm-52249R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
IF(ICC) staining with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded mouse testis tissue incubated with PBR (1D4) Monoclonal Antibody (bsm-52249R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.