bsm-52251R [Primary Antibody]
VDAC1 (9A7 ) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VDAC1

Immunogen Range: 1-100/283


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 7416

Swiss Prot: P21796

Source: Human VDAC1 protein between 1 to 100 amino acids

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.

Storage: Store at -20C for 12 months.

Background:

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Raji; Lane 2: HepG2; Lane 3: SW480; Probed with VDAC1 (9A7 ) Monoclonal Antibody (bsm-52251R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Human Raji cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Mouse Heart lysates; Lane 9: Rat Heart lysates; Lane 10: Mouse Liver lysates; Lane 11: Rat Liver lysates; Lane 12: Mouse Kidney lysates; Lane 13: Rat Kidney lysates; Lane 14: Mouse Muscle lysates probed with VDAC1 (9A7 ) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.