VALIDATION IMAGES
Lane 1: Raji; Lane 2: HepG2; Lane 3: SW480; Probed with VDAC1 (9A7 ) Monoclonal Antibody (bsm-52251R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with VDAC1 (9A7) Monoclonal Antibody (bsm-52251R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human Raji cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human SW480 cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human Jurkat cell lysates; Lane 6: Human 293T cell lysates; Lane 7: Human SH-SY5Y cell lysates; Lane 8: Mouse Heart lysates; Lane 9: Rat Heart lysates; Lane 10: Mouse Liver lysates; Lane 11: Rat Liver lysates; Lane 12: Mouse Kidney lysates; Lane 13: Rat Kidney lysates; Lane 14: Mouse Muscle lysates probed with VDAC1 (9A7 ) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VDAC1 (9A7) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, DAB staining.
