bsm-52256R [Primary Antibody]
MLKL (3G4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MLKL

Immunogen Range: 350-471/471


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 197259

Swiss Prot: Q8NB16

Source: Human MLKL between 350 to C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed:29883610).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xing, Jing. et al. CircZNF644 aggravates lipopolysaccharide-induced HK-2 cell impairment via the miR-140-5p/MLKL axis. J BIOENERG BIOMEMBR. 2022 Aug;:1-12Read more>>
VALIDATION IMAGES

Lane 1: HT29; Lane 2: HUVEC; Lane 3: Hela lysate; Probed with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.