bsm-52256R [Primary Antibody]
MLKL Recombinant Antibody
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Host: Rabbit

Target Protein: MLKL

Immunogen Range: 350-471/471


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 197259

Swiss Prot: Q8NB16

Source: Human MLKL between 350 to C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed:29883610).

Size: 100µL

Concentration: 1mg/ml

Applications: WB(1:500-2000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xing, Jing. et al. CircZNF644 aggravates lipopolysaccharide-induced HK-2 cell impairment via the miR-140-5p/MLKL axis. J BIOENERG BIOMEMBR. 2022 Aug;:1-12Read more>>
  • Dan Zhao. et al. Copper exposure induces inflammation and PANoptosis through the TLR4/NF-B signaling pathway, leading to testicular damage and impaired spermatogenesis in Wilson disease. CHEM-BIOL INTERACT. 2024 Jun;396:11106Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with MLKL (3G4) Monoclonal Antibody (bsm-52256R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


25 ug total protein per lane of various lysates (see on figure) probed with MLKL monoclonal antibody, unconjugated (bsm-52256R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MLKL Monoclonal Antibody, Unconjugated (bsm-52256R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.