bsm-52259R-RBITC

DATASHEET

Host: Rabbit

Target Protein: ERK1/2

Clonality: Recombinant

Isotype: IgG

Swiss Prot: P27361, P28482

Source: Recombinant Human ERK1 + ERK2 between 150 amino acids to C-terminus

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

p44/42 MAP Kinase(Thr202); ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Conjugation: RBITC

Excitation/ Emission: 570nm/595nm

Size: 100µL

Concentration: Lot dependent

Applications: WB(WB(1:1000-5000))
FCM(FCM(1ug/Test))
IF(IHC-P)(IF(IHC-P)(1:200-800))
IF(ICC)(IF(ICC)(1:100-500))

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.