bsm-52262R-BF488 [Conjugated Primary Antibody]
GAPDH Recombinant Antibody, AbBy Fluor-488 Conjugated
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Host: Rabbit

Target Protein: GAPDH

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 2597

Swiss Prot: P04406

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Conjugation: AbBy Fluor-488

Excitation/ Emission: 499nm/519nm

Size: 100µL

Concentration: 1mg/ml

Applications: WB(WB(1:5000-500000))
FCM(FCM(1ug/Test))
IF(IHC-P)(IF(IHC-P)(1:200-1000))
IF(ICC)(IF(ICC)(1:100-500))

Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Zebrafish
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jing Feng. et al. Insulin growth factor?1 promotes the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells through the Wnt/?catenin pathway. Exp Ther Med. 2021 Aug;22(2):1-8Read more>>
  • Li Li. et al. Circ_LPAR3 promotes the progression of oral squamous cell carcinoma (OSCC). Biochem Bioph Res Co. 2021 DecRead more>>
  • Chao Wang. et al. Inhibition of monoacylglycerol lipase restrains proliferation, migration, invasion, tumor growth and induces apoptosis in cervical cancer. 2021 Dec 07Read more>>
  • Ma, Wenbin. et al. CircPCNX Promotes PDGF-BB-Induced Proliferation and Migration of Human Aortic Vascular Smooth Muscle Cells Through Regulating miR-1278/DNMT1 Axis. CARDIOVASC DRUG THER. 2022 Jun;:1-13Read more>>
  • Feiyang Ma. et al. New insights into the interaction between duodenal toxicity and microbiota disorder under copper exposure in chicken: Involving in endoplasmic reticulum stress and mitochondrial toxicity. CHEM-BIOL INTERACT. 2022 Oct;366:110132Read more>>
  • Dr. Jing Tian. et al. Downregulation of SOX21-AS1 alleviated cisplatin resistance in cervical cancer through Epithelial-messenchymal transition (EMT) inhibition. REJUV RES. 2022 Aug 3Read more>>
  • Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 OcRead more>>
  • Yanyan Ma. et al. Sevoflurane Improves Ventricular Conduction by Exosomes Derived from Rat Cardiac Fibroblasts After Hypothermic Global Ischemia-Reperfusion Injury. DRUG DES DEV THER. 2023 Jun 11Read more>>
  • Yuan Li. et al. A Biomimetic Peptide Functions as Specific Extracellular Matrix for Quiescence of Stem Cells against Intervertebral Disc Degeneration. SMALL. 2023 Jul;:2300578Read more>>
  • Xunhu Gu. et al. METTL14-Mediated m6A Modification of TUG1 Represses Ferroptosis in Alzheimer's Disease via Inhibiting GDF15 Ubiquitination. FRONT BIOSCI-LANDMRK. 2024 Aug;29(8):298Read more>>
VALIDATION IMAGES

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GAPDH) monoclonal Antibody,FITC conjugated (bsm-52262R-BF488) 1:100, 90 min at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): BF488 conjugated Rabbit Anti-GAPDH antibody (bsm-52262R-BF488,1:100);Isotype Control (orange): Rabbit IgG/BF488 (bs-0295P-BF488). Blank control (black): PBS. Acquisition of 20,000 events was performed.