bsm-52265R [Primary Antibody]
Integrin alpha-6 (4F7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ITGA6

Immunogen Range: 1-100/1130


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3655

Swiss Prot: P23229

Source: Recombinant Human Integrin alpha 6 between 1 to 100 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion. Integrin alpha-6/beta-4 (ITGA6:ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling. ITGA6:ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 126


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Raji cell lysates probed with Integrin alpha 6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Integrin alpha-6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Integrin alpha-6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with Integrin alpha-6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Integrin alpha-6 (4F7) Monoclonal Antibody, Unconjugated (bsm-52265R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Integrin alpha-6 Monoclonal Antibody, Unconjugated (bsm-52265R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.