VALIDATION IMAGES
Raji cell lysates probed with Integrin alpha 6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Integrin alpha-6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Integrin alpha-6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with Integrin alpha-6 (4F7) Monoclonal Antibody (bsm-52265R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Integrin alpha-6 (4F7) Monoclonal Antibody, Unconjugated (bsm-52265R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Integrin alpha-6 Monoclonal Antibody, Unconjugated (bsm-52265R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.