bsm-52267R [Primary Antibody]
Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: Histone H1.3 (T17)+Histone H1.4 (T17)

Modification Site: T17+Histone H1.4

Clonality: Monoclonal

Isotype: IgG

Swiss Prot: P10412

Source: Synthetic phospho-peptide around Thr17 of human H1.4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Untreated CRC whole cell lysates; Lane 2: CRC cells treated with 1.5µg/ml Colcemid for 12 hours whole cell lysates; Probed with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody (bsm-52267R) at 1:500, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.