bsm-52268R [Primary Antibody]
NeuN Recombinant Antibody
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Host: Rabbit

Target Protein: NeuN

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 146713

Swiss Prot: A6NFN3

Source: Human NeuN between 1-100 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.

Size: 100ul

Concentration: Lot Dependent

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates probed with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN (12C3 ) ) Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Eye; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NeuN Monoclonal Antibody, Unconjugated (bsm-52268R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red, bs-0295G-BF594), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum. Merged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) . DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum. Merged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) . DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum. Merged staining of anti-GFAP (bsm-42001R; 1:200; pink) anti-AIF1/Iba1 (bsm-54132R; 1:200; red) anti-NeuN (bsm-52268R; 1:200; green) anti-MBP (bsm-33932M; 1:200; yellow) and anti-Olig2 (bsm-61115R; 1:200; light blue) . DAPI (dark blue) was used as a nuclear counter stain.