bsm-52270R
[Primary Antibody]
DUSP6 (9C3 ) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: DUSP6
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 1848
Swiss Prot: Q16828
Source: Recombinant protein within Human DUSP6 aa 1-350
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:9858808). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity).
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Mouse pancreas lysates probed with DUSP6 (9C3 ) Monoclonal Antibody (bsm-52270R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of NIH/3T3 cells with DUSP6 (9C3 ) Monoclonal Antibody (bsm-52270R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with DUSP6 (9C3) Monoclonal Antibody (bsm-52270R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with DUSP6 (9C3) Monoclonal Antibody (bsm-52270R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human stomach cancer tissue incubated with DUSP6 (9C3) Monoclonal Antibody (bsm-52270R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with DUSP6 (9C3) Monoclonal Antibody (bsm-52270R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with DUSP6 (9C3) Monoclonal Antibody (bsm-52270R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (DUSP6 (9C3) )Monoclonal Antibody, Unconjugated (bsm-52270R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
PC12 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (DUSP6 (9C3) )Monoclonal Antibody, Unconjugated (bsm-52270R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with ( DUSP6 (9C3)) Monoclonal Antibody, Unconjugated (bsm-52270R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Flow cytometric analysis of DUSP6 was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-52270R, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Lane 1: Mouse Brain Lysates; Lane 2: Mouse Pancrease Lysates; Lane 3: Mouse Liver Lysates; Lane 4: Mouse NIH/3T3 cell Lysates; Lane 5: Rat Brain Lysates; Lane 6: Rat Pancrease Lysates; Lane 7: Human HUVEC cell Lysates; Lane 8: Human U-87 MG cell Lysates; Lane 9: Human SH-SY5Y cell Lysates. Probed with DUSP6 monoclonal Antibody, unconjugated (bsm-52270R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.