bsm-52276R [Primary Antibody]
PUMA (2G6) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PUMA

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 27113

Swiss Prot: Q9BXH1

Source: Human PUMA from 150 amino acids to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391). Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis (PubMed:23340338).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela; Lane 2: K562 lysate; Probed with PUMA (2G6) Monoclonal Antibody, Unconjugated (bsm-52276R) at 1:1000 overnight at 4°C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C.


Flow cytometric analysis of HeLa cells with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse small intestine tissue incubated with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human stomach carcinoma tissue incubated with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with PUMA (2G6) Monoclonal Antibody (bsm-52276R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.