bsm-52281R [Primary Antibody]
Caspase 2 (2B1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CASP2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 835

Swiss Prot: P42575

Source: Recombinant human Caspase-2 between 150 to 350 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 48/30/12 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

293 cell lysates probed with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of Jurkat cells with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Caspase 2 (2B1) Monoclonal Antibody (bsm-52281R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.