bsm-52282R
[Primary Antibody]
Prohibitin (1F10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Prohibitin
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5245
Swiss Prot: P35232
Source: N terminal Human Prohibitin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation. Prohibitin mRNA and protein levels are high in G1, decline during the S phase, rise again in G2 and decline in M phase, which suggests that prohibitin controls the cell cycle by using both transcriptional and posttranslational mechanisms. Prohibitin is also a potential tumor suppressor protein that binds to retinoblastoma (Rb) and subsequently inhibits the activity of E2F family members in response to specific signaling cascades. Prohibitin 2 is a repressor of estrogen receptor activity, and is required for somatic and germline differentiation in the larval gonad during embryonic development. Mutations in the Prohibitin genes are correlated with breast cancer development and/or progressionin more than 80% of the cell lines analyzed.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 30
Cross Reactive Species:
Human
Mouse
Rat
Fish
Zebrafish
(Crucian carp (Carassius carassius))
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: 293; Lane 2: Jurkat; Lane 3: Mouse kidney; Lane 4: HepG2 lysate probed with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of HepG2 cells with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Hybrid fish (crucian-carp) liver tissue probed with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.