VALIDATION IMAGES
Lane 1: HepG2; Lane 2: Hela; Lane 3: NIH/3T3; Lane 4: PC-12
lysate probed with HSPA8 (4D2) Monoclonal Antibody (bsm-52284R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with HSPA8 (4D2) Monoclonal Antibody (bsm-52284R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with HSPA8 (4D2) Monoclonal Antibody (bsm-52284R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with HSPA8 (4D2) Monoclonal Antibody (bsm-52284R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with HSPA8 (4D2) Monoclonal Antibody (bsm-52284R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.