bsm-52285R [Primary Antibody]
Histone H2A (acetyl K9) (4G2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Histone H2A(acetyl K9)

Modification Site: acetyl K9

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3012, 8335

Swiss Prot: P04908

Source: Synthetic peptide surrounding K9 of Histone H2A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 14


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HeLa treated with 500 ng/ml Trichostatin A for 4 hours whole cell lysates; Lane 2: Untreated HeLa whole cell lysates probed with Histone H2A(acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:500 and unmodified Histone H2A.X antibody as control, incubated overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human lung carcinoma tissue incubated with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse thyroid tissue incubated with Histone H2A (acetyl K9) (4G2) Monoclonal Antibody (bsm-52285R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.