bsm-52290R
[Primary Antibody]
beta Tubulin (2F11) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: beta Tubulin
Clonality: Monoclonal
Isotype: IgG
Swiss Prot: P07437
Source: Human beta Tubulin aa 300 to the C-terminus
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Yingying Che. et al. Splicing factor SRSF3 promotes the progression of cervical cancer through regulating DDX5. MOL CARCINOGEN. 2022 OcRead more>>
VALIDATION IMAGES
Lane 1: Hela; Lane 2: NIH/3T3; Lane 3: PC12 lysate probed with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of NIH/3T3 cells with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
IF(ICC) staining with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with beta Tubulin (2F11) Monoclonal Antibody (bsm-52290R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human 293T cell lysates probed with beta Tubulin Monoclonal Antibody, Unconjugated (bsm-52290R) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.