bsm-52297R
[Primary Antibody]
Active Caspase 3 (2F7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Active Caspase-3
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 836
Swiss Prot: P42574
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 17
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Camptothecin (2 µM) treated Jurkat cells; Lane 2: Untreated Jurkat cell lysate probed with Active Caspase-3 (2F7) Monoclonal Antibody (bsm-52297R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.