bsm-52302R [Primary Antibody]
ARPC5 Recombinant Antibody
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Host: Rabbit

Target Protein: ARPC5

Immunogen Range: 1-50/151


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10092

Swiss Prot: O15511

Source: Recombinant human p16 ARC around N-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


25 ug total protein per lane of various lysates (see on figure) probed with ARPC5 monoclonal antibody, unconjugated (bsm-52302R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.