bsm-52302R [Primary Antibody]
ARPC5 Recombinant Antibody
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Host: Rabbit

Target Protein: ARPC5

Immunogen Range: 1-50/151


Clonality: Recombinant

Isotype: IgG

Entrez Gene: 10092

Swiss Prot: O15511

Source: Recombinant human p16 ARC around N-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Size: 100uL, 20uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(1:50-200)
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Predicted Molecular Weight: 16


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with ARPC5 monoclonal antibody, unconjugated (bsm-52302R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ARPC5 Monoclonal Antibody, Unconjugated (bsm-52302R) at 1:200 overnight at 4°C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.