bsm-52304R
[Primary Antibody]
BCL2 (5F7) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: BCL2
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 596
Swiss Prot: P10415
Source: Recombinant human Bcl-2 between 1 to 200 amino acids.
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Size: 100ul
Concentration: 1ug/ul
Predicted Molecular Weight: 26/22 kDa
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang, Sanchun. et al. Pseudoginsengenin DQ exerts antitumour activity against hypopharyngeal cancer cells by targeting the HIF-1-GLUT1 pathway. Cancer Cell Int. 2021 Dec;21(1):1-12Read more>>
- Yu-Sheng Shi. et al. Pteris laeta Wall. and Its New Phytochemical, Pterosinsade A, Promote Hippocampal Neurogenesis via Activating the Wnt Signaling Pathway. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
- Lisha Peng. et al. miR-128-3p increases the radiosensitivity in nasopharyngeal carcinoma via regulating vascular endothelial growth factor C. PATHOL RES PRACT. 2023 Aug;:154768Read more>>
- Zhang Zhichun. et al. A novel SLC25A1 inhibitor, parthenolide, suppresses the growth and stemness of liver cancer stem cells with metabolic vulnerability. CELL DEATH DISCOV. 2023 Sep;9(1):1-12Read more>>
- Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
- Di Zhao. et al. Protective effect of exosomes derived from bone marrow mesenchymal stem cells on hypoxia reperfusion injury of cardiomyocytes. CELL MOL BIOL. 2024 Feb;70(2):73-8Read more>>
VALIDATION IMAGES
Lane 1: Hela; Lane 2: Jurkat lysate probed with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of Jurkat cells with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Jurkat cell lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bsm-52304R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with BCL2 (5F7) Monoclonal Antibody (bsm-52304R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BCL2 (5F7) Monoclonal Antibody(bs-52304R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human HeLa cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human THP-1 cell lysates probed with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-52304R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.