bsm-52304R [Primary Antibody]
Bcl-2 Recombinant Antibody
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Host: Rabbit

Target Protein: Bcl-2

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 596

Swiss Prot: P10415

Source: Recombinant human Bcl-2 between 1 to 200 amino acids.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)

Predicted Molecular Weight: 26/22 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Sanchun. et al. Pseudoginsengenin DQ exerts antitumour activity against hypopharyngeal cancer cells by targeting the HIF-1-GLUT1 pathway. Cancer Cell Int. 2021 Dec;21(1):1-12Read more>>
  • Yu-Sheng Shi. et al. Pteris laeta Wall. and Its New Phytochemical, Pterosinsade A, Promote Hippocampal Neurogenesis via Activating the Wnt Signaling Pathway. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Lisha Peng. et al. miR-128-3p increases the radiosensitivity in nasopharyngeal carcinoma via regulating vascular endothelial growth factor C. PATHOL RES PRACT. 2023 Aug;:154768Read more>>
  • Zhang Zhichun. et al. A novel SLC25A1 inhibitor, parthenolide, suppresses the growth and stemness of liver cancer stem cells with metabolic vulnerability. CELL DEATH DISCOV. 2023 Sep;9(1):1-12Read more>>
  • Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
  • Di Zhao. et al. Protective effect of exosomes derived from bone marrow mesenchymal stem cells on hypoxia reperfusion injury of cardiomyocytes. CELL MOL BIOL. 2024 Feb;70(2):73-8Read more>>
  • Lei Liu. et al. Myricetin Oligomer Triggers Multi-Receptor Mediated Penetration and Autophagic Restoration of Blood-Brain Barrier for Ischemic Stroke Treatment. ACS NANO. 2024;XXXX(XXX):XXX-XXXRead more>>
  • ang-Hua Zhong. et al. Transcription factor FOXF2 promotes the development and progression of pancreatic cancer by targeting MSI2. ONCOL REP. 2024 Jul;52(1):1-13Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Bcl-2 monoclonal antibody, unconjugated (bsm-52304R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.